Gold Biotechnology, Inc.
Gold Biotechnology, Inc.
  • Видео 59
  • Просмотров 1 114 739
How to Choose the Right Competent Cells for your Experiment
In this video, we delve into the complexities of selecting the right competent cells for research applications, focusing primarily on E. coli strains. We explore key considerations such as equipment availability, transformation efficiency, budget constraints, and specific cell requirements. Whether you're prioritizing high transformation efficiency or specific genotype characteristics, we'll help you find the the right competent cells for your experiment.
More Resources
How to Choose Competent Cells - goldbio.com/articles/article/how-to-choose-competent-cells
GoldBio Competent Cells Characteristics and Applications - goldbio.com/documents/4076/GoldBio%20Competent%20Cells%20Characteristic%2...
Просмотров: 17

Видео

GB5-alpha and GB10B: E. coli Cloning Strains 101
Просмотров 2714 дней назад
Welcome to our in-depth look at E. coli cloning strains! In this video, we spotlight GB5-alpha and GB10B cells, highlighting their mutations and capabilities that make them perfect for cloning. We'll discuss how these strains prevent DNA degradation, support large vector cloning, and allow for effective blue-white screening. Ideal for both beginners and experienced researchers, this video is yo...
Tips for Choosing Between Chemical Transformation and Electroporation
Просмотров 25Месяц назад
In this video, we compare two common methods for cell transformation: electroporation and chemical transformation (heat shock). We'll compare the pros and cons of each method to help you decide which is best for your lab work. More Resources Tips for Choosing Between Heat Shock or Electroporation - goldbio.com/articles/article/Choosing-Between-Heat-Shock-or-Electroporation How to Choose Compete...
Finally Understand Alpha Complementation and Blue-White Screening (Part 1)
Просмотров 3194 месяца назад
In this video, we'll break down the complexity of alpha-complementation by starting with a simple analogy that anyone can grasp. No need to worry about diving too deep into the science at the beginning - we'll gradually explore the technical aspects and connect them back to our easy-to-understand analogy. Then in part two we'll explain how this plays a part in blue-white screening. Part Two: ru...
Blue-White Screening Demystified (Part 2 )
Просмотров 1164 месяца назад
In part one, we delved into alpha-complementation using a few analogies that made the concept easy for anyone to understand. Now, in part two, we're taking that understanding further by exploring how alpha-complementation ties into blue-white screening. Part One: ruclips.net/video/h9ndu9-H758/видео.html More Resources Explain Alpha-Complementation and Blue-White Screening To Me Like I’m 10 - go...
SDS-PAGE Gel Casting: Common Problems & Fixes
Просмотров 3317 месяцев назад
Having problems casting SDS-PAGE gels? Often there are three signs that indicate issues with acrylamide gel casting: bands not being parallel, samples leaking out of the well while loading wells, and bands not separating properly. Indicators of SDS-PAGE Issues: 0:35 - Bands Are Not Parallel 1:44 - Samples Leaking Out 2:30 - Bands Not Separating More Resources Troubleshooting Gel Casting/ Polyme...
From Smearing to Smiling: SDS-PAGE Troubleshooting Tips
Просмотров 1,6 тыс.9 месяцев назад
Are you having problems running your SDS-PAGE gels? Discover the answers you've been looking for in this in-depth video on troubleshooting SDS-PAGE gel running issues. We break down six common issues while running your protein sample in an SDS-PAGE gel, including protein sample migration, smearing, and improper separation. For each issue we will provide analytical insight into what might have g...
Clean Up Your Laboratory Fridge/Freezer
Просмотров 75611 месяцев назад
Keeping your laboratory fridges and freezers organized is essential for efficiency and safety. When the door is open for too long, it wastes energy and can compromise the quality of the items stored inside. This guide will show you how to organize your fridges and freezers so that you can find what you need quickly and easily, while also keeping your samples and reagents safe. Resources 10 Easy...
How to make 1L of 1M HEPES Buffer
Просмотров 1,3 тыс.Год назад
A quick protocol for making a sterile stock buffer with HEPES Free Acid. Protocol used - goldbio.com/documents/3548/HEPES Buffer 1M Stock Solution with table.pdf More Resources GoldBio Buffers Handbook: Download for Free - goldbio.com/goldbio-buffers-handbook-download-for-free What is a Biological Buffer and How to Choose the Best Buffer for Your Experiment - goldbio.com/articles/article/what-i...
What do Firefly Flashes Mean?
Просмотров 444Год назад
Today, many of us have fond memories of lightning bugs from our childhood summers. But do you know what those flashing lights mean? Resources The Secret Codes Embedded In Firefly Flashes - goldbio.com/articles/article/Secret-Codes-Embedded-In-Firefly-Flashes Written, narrated and edited by Karen Pierce Gold Biotechnology, Inc. - www.goldbio.com At GoldBio, we strive to exceed the expectations o...
15 Great Biological Discoveries that Revolutionized Life Science
Просмотров 1,3 тыс.Год назад
Learn about the fifteen most revolutionizing discoveries in biology. Advancements in the origins of life, the mechanisms of life, cures, new (and some rediscovered) maladies - each of these innovations propelled the field forward into the future. Here we present 15 breakthroughs that changed the course of the history of biology. Chapters: Inheritance/Evolution 0:53 Antibiotics: 1:35 Gel Electro...
Quick Guide to Pronouncing Biological Buffers
Просмотров 433Год назад
Here is a quick guide to how to correctly say some of the most common buffers in the lab For more information on biological buffers along with illustrations, protocols and tips to help ensure your experiment is a success check out our GoldBio Buffers Handbook: www.goldbio.com/goldbio-buffers-handbook-download-for-free Acronyms vs initialisms - 0:26 Buffer initialisms - 1:12 ADA - 1:14 BES - 1:1...
A Crash Course on Luciferase Assays
Просмотров 9 тыс.Год назад
Here is a quick guide to some of the most asked questions about running a Luciferase Assay for the first time. For more information along with illustrations, protocols and tips to help ensure your experiment is a success check out our GoldBio Luciferin In Vitro Handbook - www.goldbio.com/goldbio-luciferin-in-vitro-handbook-download-for-free Questions Answered: What is the luciferin/luciferase r...
Lab Break: Why Are Puppy Dog Eyes So Effective?
Просмотров 3232 года назад
Why do puppy dog eyes make our hearts melt? Learn about the science behind this cute expression and how dogs gained this adorable superpower. Resources Irresistible Puppy Eyes and Their Science - www.goldbio.com/articles/article/Irresistible-Puppy-Eyes-And-Their-Science Written by Karen Martin; Narrated and edited by Karen Pierce Gold Biotechnology, Inc. - www.goldbio.com At GoldBio, we strive ...
Lab Break: Can plants think?
Просмотров 2512 года назад
This video explores the ideas and questions behind plant intelligence. How do biologists define intelligence? How do plants interact to their environment? Can an organism express intelligent behavior without having a brain? Find out in this short video based off of one of our articles Resources Can Plants Think? - www.goldbio.com/articles/article/Can-Plants-Think Written by Karen Martin; Narrat...
Prueba de antibiótico mediante el ensayo de difusión en disco: Método de Kirby Bauer
Просмотров 6 тыс.2 года назад
Prueba de antibiótico mediante el ensayo de difusión en disco: Método de Kirby Bauer
Cómo Intepretar Resultados De Electroforesis En Gel: Diferentes tipos de plásmido de ADN
Просмотров 13 тыс.2 года назад
Cómo Intepretar Resultados De Electroforesis En Gel: Diferentes tipos de plásmido de ADN
Cómo hacer una PCR
Просмотров 1,2 тыс.2 года назад
Cómo hacer una PCR
Tips For Writing the Discussion Section of your Research Manuscript
Просмотров 4922 года назад
Tips For Writing the Discussion Section of your Research Manuscript
Tips for Writing the Results Section of your Manuscript
Просмотров 4822 года назад
Tips for Writing the Results Section of your Manuscript
How to Green Your Lab 10 Sustainable Things Every Lab Can Do
Просмотров 4972 года назад
How to Green Your Lab 10 Sustainable Things Every Lab Can Do
10 Consejos para Conservar un Buen Cuaderno de Laboratorio
Просмотров 1,1 тыс.2 года назад
10 Consejos para Conservar un Buen Cuaderno de Laboratorio
How to Detect Cell Culture Contamination
Просмотров 13 тыс.2 года назад
How to Detect Cell Culture Contamination
New Laboratory Setup - Panel Q & A With Academic, Industry, Teaching and Sustainability Experts.
Просмотров 8722 года назад
New Laboratory Setup - Panel Q & A With Academic, Industry, Teaching and Sustainability Experts.
Choose the Right Chart to Showcase Your Data
Просмотров 4443 года назад
Choose the Right Chart to Showcase Your Data
Preattentive Attributes: Make Data Stand Out
Просмотров 7433 года назад
Preattentive Attributes: Make Data Stand Out
Basics of Figures and Tables for Your Research Publication
Просмотров 4 тыс.3 года назад
Basics of Figures and Tables for Your Research Publication
Differentiate Large And Small Strands Of DNA: Interpreting Gel Electrophoresis Results
Просмотров 2,1 тыс.3 года назад
Differentiate Large And Small Strands Of DNA: Interpreting Gel Electrophoresis Results
How to Interpret Gel Electrophoresis Results: Different types of plasmid DNA
Просмотров 33 тыс.3 года назад
How to Interpret Gel Electrophoresis Results: Different types of plasmid DNA
Why Kinetic Curves Are Essential for Bioluminescence Imaging
Просмотров 3163 года назад
Why Kinetic Curves Are Essential for Bioluminescence Imaging

Комментарии

  • @dsdeshmukh4024
    @dsdeshmukh4024 27 дней назад

    what are broad spectrum antibiotics example like ciprofloxacin? ,which one solvent has high impact with amphotericin b DMSO or Water?

    • @kosheeka
      @kosheeka 21 день назад

      Ciprofloxacin is a commonly used broad-spectrum antibiotic in cell culture that can be replaced with gentamycin or tobramycin. DMSO is the preferred solvent for dissolving amphotericin B in cell culture applications. It has a high capacity to solubilize the compound. Amphotericin B has very low solubility in water. Using water can lead to aggregation and precipitation, making it unsuitable for cell culture experiments.

  • @kristinaanugerahaji9220
    @kristinaanugerahaji9220 Месяц назад

    Excuse me, may I use this video as an open learning resource/supplement to learning material at the Open University? Thank you for the permission granted.

    • @Goldbio
      @Goldbio Месяц назад

      Hi there. If you're using the link where it will direct people to this RUclips video, absolutely. And we would definitely appreciate the credit too. However, if it's uploading a video onto a specific platform outside of RUclips, then we would not allow that. I believe embedding from RUclips is OK since it's still basically on the RUclips platform in the end, if that makes sense.

  • @reyhaneesmaieli1117
    @reyhaneesmaieli1117 Месяц назад

    this was soooo helpful. thank you

  • @eddiejenkins7613
    @eddiejenkins7613 Месяц назад

    How about the brain How can the FGF1 HELP THE BRAIN

  • @priyankanilangekar3161
    @priyankanilangekar3161 2 месяца назад

    How to sterile that filter paper discs

  • @El.moomin.esquizoide
    @El.moomin.esquizoide 2 месяца назад

    EXPLICACION PRECISA, CLARA. GRACIAS

  • @uquantum
    @uquantum 2 месяца назад

    So useful! Loved the tips about wider margins, and the time lock (daily time) so it gets updated and reviewed daily.

  • @tarikabaraka2251
    @tarikabaraka2251 4 месяца назад

    Un cuaderno de laboratorio o libro de laboratorio es un registro primario de investigación. Los investigadores usan un cuaderno de laboratorio para documentar sus hipótesis, experimentos y análisis o interpretación inicial de estos experimentos.

  • @BBoldGaming
    @BBoldGaming 6 месяцев назад

    This should have waaaaaay more views. 📌

  • @pegahsarraf4109
    @pegahsarraf4109 6 месяцев назад

    thank you! it was informative.

  • @nadasalah8455
    @nadasalah8455 6 месяцев назад

    Thanks alot❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤❤

  • @mushtaqahmad3020
    @mushtaqahmad3020 7 месяцев назад

    Respect from Pakistan.🥰

  • @sandraerdmann6003
    @sandraerdmann6003 8 месяцев назад

    Great video. I just have on comment on negative controls. What you've meant is a no-template control. A negative control contains DNA that is the "wrong" template for your primers, for example from a different species, that doesn't contain that target gene region.

  • @aarjvijain
    @aarjvijain 8 месяцев назад

    I hate how scientists named Dh5 alpha as alpha even though it DOES NOT have alpha fragment. I always forget where the alpha and Omega genes are.😅😤

  • @anishagulgule04
    @anishagulgule04 8 месяцев назад

    This quest was asked in viva.

  • @maritaalvarez9794
    @maritaalvarez9794 8 месяцев назад

    Y de poliacrilamida seria lo mismo en gel de agarosa?

  • @anacristinademartinez3495
    @anacristinademartinez3495 9 месяцев назад

    ¡GRACIAS!

  • @curiousguy4
    @curiousguy4 10 месяцев назад

    Can you please make a video on identification of PCR products bands using SSR markers because ssr marker makes multiple bands which are difficult to analyse

  • @user-nj1bw4jm6q
    @user-nj1bw4jm6q 11 месяцев назад

    This video is awesome. We need this kind of united pronunciation.

  • @funny11744
    @funny11744 11 месяцев назад

    I like presentation.

  • @connorgoldy7706
    @connorgoldy7706 Год назад

    Thank you this was helpful!

  • @user-pw1dl9vo3x
    @user-pw1dl9vo3x Год назад

    Good job madam ❤ thank you

  • @user-pw1dl9vo3x
    @user-pw1dl9vo3x Год назад

    Good job madam ❤ thank you

  • @reza310
    @reza310 Год назад

    Very helpful thank you.

  • @latetodagame1892
    @latetodagame1892 Год назад

    I think the professor I worked under poured a glass of wine before she'd write a long entry. 🍷😂🎉

  • @HariPrasad-xs4kw
    @HariPrasad-xs4kw Год назад

    Does ladder, Marker and bands are same if not what is the difference?

  • @nurulaliana5956
    @nurulaliana5956 Год назад

    how do you sterilize the filter paper disc?

  • @user-bn6nu1do8y
    @user-bn6nu1do8y Год назад

    awesome

  • @hannahgallichan238
    @hannahgallichan238 Год назад

    When he explains better than my prof that is world famous in this field>>>>>

  • @bubblebaath7840
    @bubblebaath7840 Год назад

    I'm reading a paper right now and he mentioned using a "plate-hole diffusion" assay to test his extract against a number of gram positive bacteria, I cant find anywhere that mentions an assay technique by that name though.. Ima just pretend he used this exact technique and call it a day bc im tired of scrolling through google nvm i just remembered i can put quotation marks in google search and only show results with exactly what i search for, we chillin now

  • @jacksonmulwa9406
    @jacksonmulwa9406 Год назад

    Exactly what I want

  • @girish869
    @girish869 Год назад

    I'm looking for information regarding chemical contamination related cell behaviour.

    • @kosheeka
      @kosheeka 17 дней назад

      Chemical contamination can be a real challenge, not just from bacterial growth but also from unexpected sources. Improper media preparation, degraded components over time, or even chemicals leaching from cultureware can all disrupt cell behavior. This can lead to misleading results in experiments, where the cells are stressed or not functioning normally. Your video does a great job of highlighting the importance of maintaining a clean and controlled environment for these finicky cells. If you have any specific question in mind, please feel free to ask.

  • @fabioromano7643
    @fabioromano7643 Год назад

    Molto inspirante

  • @fishingwithlogi9858
    @fishingwithlogi9858 Год назад

    Thank you so much, this video was supper helpful.

  • @WooDong
    @WooDong Год назад

    화이팅 😀

  • @shinyanand9000
    @shinyanand9000 Год назад

    Which media is used to identify?

  • @renassalahudden9520
    @renassalahudden9520 Год назад

    thanks so much

  • @hebaomar3001
    @hebaomar3001 Год назад

    Thank you 🙏

  • @byankezia6798
    @byankezia6798 Год назад

    Where do we mixing master mix? Laminary flow?

  • @Chicken-or-watermelon
    @Chicken-or-watermelon Год назад

    Thanks! Started my final year project this week and was hoping to get everything right from the get-go.

  • @imanalansary4538
    @imanalansary4538 Год назад

    Thanks for your explanations ❤

  • @SwadhinMeher-us8ch
    @SwadhinMeher-us8ch Год назад

    Great video to get started. Thank you

  • @sarashamseldin9864
    @sarashamseldin9864 Год назад

    Can I use the disc immediately after adding the antibiotic without drying ??And thanks alot :-)

  • @quirma6471
    @quirma6471 Год назад

    hi can yall reply to me the materials that were said in the video? tenksss

    • @Goldbio
      @Goldbio Год назад

      Good morning, here is the link to the protocol: www.goldbio.com/documents/1329/GoldBio%20Disk%20Diffusion%20Assay%20Protocol.pdf Materials needed are:  Antibiotic to be tested  Control Antibiotic (either previously tested product or commercially available disk)  1 overnight culture of resistant strain  1 overnight culture of susceptible strain  6 mm Whatman filter paper disks  Sterile petri dishes  2 Agar plates (with no antibiotic)  Tweezers

    • @Goldbio
      @Goldbio Год назад

      Additionally, from the video description, here are some of the specific materials: -6 mm filter paper discs -sterile petri dishes -agar plates with no antibiotic -sensitive and resistant bacterial cultures -antibiotic to test and antibiotic for control

  • @BioprofeCol
    @BioprofeCol Год назад

    Excelente explicación Saludos,

  • @samarakadhim288
    @samarakadhim288 2 года назад

    Very informative and helpful video alot of thanks 🌹🌹🌹

  • @umisyafiqah8054
    @umisyafiqah8054 2 года назад

    This was really helpful, thanks a lot !

  • @fantastic1368
    @fantastic1368 2 года назад

    What is use of antibiotic control ??

  • @angelicacastillogarcia6153
    @angelicacastillogarcia6153 2 года назад

    I´m really thankful for this amazing video, it was very educative and helped me a lot to discuss the results of the PCR with my classmates. Good job <3

  • @ricardomerlo4878
    @ricardomerlo4878 2 года назад

    Muchas gracias por el video. Me resultó muy útil para comprender lo necesario que es tener ese cuaderno.